human mouse cd44 conjugated  (Multi Sciences (Lianke) Biotech Co Ltd)

Journal: Journal of Pharmaceutical Analysis

Article Title: Hyaluronic acid-modified polymeric nanoplatform delivering 131 I-Hyp suppresses post-ablation residual lesions in colorectal cancer metastases via necrosis-targeted radiotherapy

doi: 10.1016/j.jpha.2025.101488

Figure Lengend Snippet: CD44 receptor expression and HA-PCL@( 131 I-Hyp) nanoparticles (HP-NPs) binding affinity in vitro . (A) Immunocytochemistry of CD44 expression in HT-29 and HCT-15 cells assessed by fluorescence microscopy with a fluorescein isothiocyanate (FITC) dye. (B) Flow cytometry analysis of CD44 surface expression in HT-29 and HCT-15 colorectal cancer cell lines. Cells were analyzed using unstained isotype controls for gating. CD44-positive cells represented 86.8% of the HT-29 population versus 0.65% of the HCT-15 population. (C) Intracellular distribution of fluorescence from hypericin (Hyp) at 0.5, 1, and 2 h posttreatment in HT-29 cells, HCT-15 cells, and HT-29 cells treated with free hyaluronic acid (HA). Red fluorescence: Hyp; blue fluorescence: Hoechst. (D, E) Flow cytometry (D) and quantitative analysis (E) of the fluorescence intensity of HT-29 and HCT-15 cells incubated with the same concentration of HP-NPs. ∗ P < 0.05, ns: no significant. HA-PCL: hyaluronan- b -poly(ε-caprolactone); DAPI: 4',6-diamidino-2-phenylindole.

Article Snippet: Anti-human/mouse CD44 conjugated with fluorescein isothiocyanate (FITC) (F1104401; Lianke Biotech Co., Ltd., Hangzhou, China) was added to the cells in the experimental groups at a 1:20 dilution in flow staining buffer, and the mixture was incubated at room temperature for 15 min in the dark.

Techniques: Expressing, Binding Assay, In Vitro, Immunocytochemistry, Fluorescence, Microscopy, Flow Cytometry, Incubation, Concentration Assay

Journal: iScience

Article Title: Cell types or cell states? An investigation of adrenergic and mesenchymal cell phenotypes in neuroblastoma

doi: 10.1016/j.isci.2024.111433

Figure Lengend Snippet: List of antibodies

Article Snippet: Human/Mouse CD44 Alexa Fluor® 647-conjugated Antibody , R&D Systems , Cat# FAB6127R; RRID: AB_3651395.

Techniques: Control

Journal: iScience

Article Title: Cell types or cell states? An investigation of adrenergic and mesenchymal cell phenotypes in neuroblastoma

doi: 10.1016/j.isci.2024.111433

Figure Lengend Snippet:

Article Snippet: Human/Mouse CD44 Alexa Fluor® 647-conjugated Antibody , R&D Systems , Cat# FAB6127R; RRID: AB_3651395.

Techniques: Control

Journal: Toxicology reports

Article Title: Silver nanoparticles induces apoptosis of cancer stem cells in head and neck cancer.

doi: 10.1016/j.toxrep.2023.11.008

Figure Lengend Snippet: Fig. 4. CSC isolated from Cal33 characterization by Flow Cytometry. Population 1 and Single cell dot plots show the gating strategy for events of interest and eliminating doublets and triplets. Untreated is a negative control for CD44+PE and CD24+APC staining. Before CSC isolation shows 55% of CD44+ and CD24 low

Article Snippet: FITC Annexin V Apoptosis Detection Kit (Cat no. 640914) was purchased from BD Bioscience, MagCellect Streptavidin Ferrofluid (Cat no. MAG999B), CD24 Biotinylated Antibody (Cat no. FAB52471B), Human CD24 APC-conjugated Antibody (Cat no. FAB5247A) and Human CD44 PE-conjugated Antibody (Cat no. FAB8547P) from R&D Systems Minneapolis, Minnesota, United States.

Techniques: Isolation, Flow Cytometry, Negative Control, Staining